Friday 15 July 2016

Running a big RNA-seq project is easy(ish)

Last year we completed our largest ever RNA-seq project: 528 samples of TruSeq mRNA, 60 lanes of HiSeq 2500 SE50, 13 billion reads - and all in 16 weeks. Being able to do such a large project in such a short time and get high quality data from nearly all samples really demonstrates the robustness of RNA-seq. If you're thinking that a project larger than 96 samples might be too much to consider, then come and talk to us (and Bioinformatics) at a Tuesday afternoon experimental design meeting - and we'll convince you it can be a pretty smooth process.



We've been using Illumina's TruSeq mRNA-seq automated on our Agilent Bravo robot and the sequencing was done on HiSeq 2500, although we're currently  moving to HiSeq 4000.
  • 528 samples processed on six-plates of RNA-seq
  • QC lanes sequenced and analysed
  • 60 lanes of SE50bp sequencing in total, 10 lanes per plate
  • 12,918,018,345 PF reads for this project (215M reads per lane on average)
  • 24M reads per sample on average
  • 16 weeks from start to finish
This has been a large and complex project where we had lots of discussions along the way. I think that everyone involved has contributed to the success so far: the research group who asked us to do the project, my lab, and also our Bioinformatics Core. The ability to discuss the experiment at different stages, and to focus on QC issues as they arise really makes using the Cores a great place to do your projects.

49 comments:

  1. Replies
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  3. i already gave up on ever getting cured of HSV2 because i have try many treatment none of them work out for me i have go to different hospital they always tell me same thing there is no cure for herpes when i came across a post about Dr ohunyom, in the net from a lady called Angela i contacted him and he reassured me with him herbal medicine which i took according to the way he instructed, that how i was cured. I doubted at first because i have been to a whole lot of reputable doctors, tried a lot of medicines but none was able to cure me. so i decided to listen to him and he commenced treatment, and under two weeks i was totally fee from #Herpes. i want to say a very big thank you to DR ohunyom for what he has done in my life. feel free to leave him a message on email drohunyom@gmail.com and also WhatsApp him +2349060579973..
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    Replies
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  10. I'm so exited to share my testimonies about the Good Work of Dr.Ehiaguna who get me cured from herpes simplex virus (HSV1&2) with his herbs, I never thought that I will live on earth before the year runs out. I have been suffering from herpes, I had spent a lot of money going from one Hospital to another looking for way to get rid of this disease, the hospital have been my home everyday residence. Constant checks up have been my hobby not until this faithful day, I was searchingthrough the internet, I saw a testimony on how Dr. Ehiaguna helped someone in curing his herpes disease using his healingHerbs, quickly I copied his email just to give him a try I spoke to him, he told me that he is going to provide the herbal cure to me, which hedid, after i receive his herbs and i take it as instructed i was cure permanently from herpes my herpes disease was gone. so I decided to share my testimony, that nothing is impossible with God, God use a man to healed me. No matter what you are passing through, no matter how deadly the sickness is and no matter what the situation is God that did mine is still going to do yours, people suffering from herpes, brain tumor, kidney disease, pcos, AIDS, ALS,copd, asthma, athritis,herpes, Cancer,Hpv, any kind of disease, you can reach him now via ? Gmail address: drehiaguna@gmail.com or whatsapp +2348073908953 and you will be the next to testify of his great work.

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  11. 5 years ago I had warts, I was treated with some liquid applied to the warts they continued to grow and spread... The next 2 doctors did laser surgery to remove them. 1 year after the surgery, they grew back close to where the 1st ones were' so I was finally told it was hpv. I have had it for very long time, I contract it from my cheated boyfriend and I found out he was also infected and I end up the relationship between us. the warts was so embarrasses because it started spreading all over I have be dealing with this things for very long time the last treatment I take was About 2 years ago I applied natural treatment from Dr onokun herbal cure, a week after applying the treatment all the warts was gone. it's now 2 years and some months I don't have single wart or any symptoms of hpv. wow"" it's great, Dr onokun has finally cured me. Anyone living with hpv contact Dr onokun for natural treatment.
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  14. I never thought i would be HIV negative again after been diagnosed in 2017, i have tried everything possible in life from one doctor to another, one hospital to another, series of tests, different kinds of medication, i had already lost hope until i meet Great Dr. OSAGIE online testimonies, a specialist in herbal medication from Africa, i contacted him (drosagiesolutiontemple@gmail.com OR
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  15. 5 years ago I had warts, I was treated with some liquid applied to the warts they continued to grow and spread... The next 2 doctors did laser surgery to remove them. 1 year after the surgery, they grew back close to where the 1st ones were' so I was finally told it was hpv. I have had it for very long time, I contract it from my cheated boyfriend and I found out he was also infected and I end up the relationship between us. the warts was so embarrasses because it started spreading all over I have be dealing with this things for very long time the last treatment I take was About 2 years ago I applied natural treatment from Dr onokun herbal cure, a week after applying the treatment all the warts was gone. it's now 2 years and some months I don't have single wart or any symptoms of hpv. wow"" it's great, Dr onokun has finally cured me. Anyone living with hpv contact Dr onokun for natural treatment.
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  17. I used to suffer from HERPES GENITAL for about 3 years so I can relate
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  18. Thank God for using Dr Ojamo to get me cured from Herpes virus
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  19. When I found Dr. OGEDEGBE I was desperate in need of bringing my ex lover back. He left me for another woman. It happened so fast and I had no say in the situation at all. He just dumped me after 3 years with no explanation. I contact Dr. OGEDEGBE through his Whats app number +2348109374702  and He told me me what i need to do before he can help me and i did what he told me to, after i provided what he wanted, he cast a love spell to help us get back together. Shortly after he did his spell, my boyfriend started texting me again and felt horrible for what he just put me through. He said that I was the most important person in his life and he knows that now. We moved in together and he was more open to me than before and then he started spending more time with me than before. Ever since Dr. OGEDEGBE helped me, my partner is very stable, faithful and closer to me than before. I highly recommends Dr. OGEDEGBE to anyone in need of help. Email: dr.ogedegbe6@gmail.com Call him or add him on whatsapp via: +2348109374702  ..........    ..........  ........

    ReplyDelete
  20. Never give up in life they all say no cure to HSV 2 which is a big lie I have pass through many process also i never believe there is really cure to HERPES until I meet Dr. Sayo the doctor that have been helping many people for many years, I come across this doctor online when I was searching for cure online I found out about this man, and to my greatest surprise this man have the herbal medicine which I have been looking for years I explain my problem to him through the email I found on someone who testify about him also, Dr.Sayo reply and explain how the process work so after ordering for the medicine I got it within 4 days and I took it according to the way Dr.Sayo instructed, I was so happy after two week I took the medicine there was very big change in my health when I was done with the process I go for test, I found out I am negative that was the day I have the tears of joy you can also get in contact with my doctor through his email now (sayoherbalhealer@gmail. com) OR Contact Sayo by check FB Page https://www.facebook.com/Sayo-Herbal-Healer-100145798345000/  check website; http://sayoherbalhealer.website2.me/  check blog: https://sayoherbalhealer.blogspot.com/  And He also have herbs medicine to cured the following diseases; eczema,urethra wart,chronic problems.Herpes, Cancer, Als,Hepatitis, Diabetes, HPV,Infections,ulcer  ETC 

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  21. Hi it's laa! 9 months ago my doctor told me I was diagnosed with herpes. I was heart broken, However that same month i found a Dr. who helped me to cured the virus with natural herbal treatment permanently. thanks to Dr. omohan for his natural herbal treatment that eliminated this virus out in my body forever. his contact information for any incurable virus via; dromohanherbalmedicine@gmail.com or whatsapp him or call him through his phone number +2348164816038.    

    ReplyDelete
  22. I have been battling this Herpes disease for almost 6 years now….I tried all possible means to get cure from my Herpes Disease but all to be in vain until i saw a post in a health forum about a herbal Dr Iyoha who prepare herbal medicine to cure all kind of diseases including Herpes Disease, at first i doubted if it was real but decided to give it a try…when i contact Dr Iyoha via his email (driyohasolutiontemple@yahoo.com) write him and reply me explain how the process work so after ordering for the medicine I got it within 3/4 working days through DHL Delivery and I took it according to the way Dr Iyoha instructed, I was so happy after 2 week I took the medicine there was very big change in my health when I was done with the process I go for test, I found out I am negative…Herpes patients should also get in touch with this herbalist Dr Iyoha to get rid of these Herpes Virus forever you can contact Dr Iyoha for help. Thanks once again Dr Iyoha for making me a happy woman again. You can contact him: driyohasolutiontemple@yahoo.com WHATSAPP: +1 8572288347 OR CALL : +1 4073379869 .

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  23. I want to share this wonderful testimony to the Good people all over the world on how I was able to Enlarge my Penis by Dr. Aziba. I was living a shameful life from my young age, just last month as I was browsing on the internet about Penis size and Enlargement Products, I saw a testimony of a Man called Tim James testifying of how he was able to get his penis Enlarged by Dr. Aziba and I decided to also Email Dr. Aziba for my small penis size and he quickly respond to me and gave me the normal instructions which i did and then he shipped the product to me here in the united state which i received in just 3 working days and today i am very happy because i started seeing positive changes in my penis size in just 7 days of use. Dr Aziba herbal product is the best recommended for you and to whom ever suffering from this shame or having any other diseases as well should Contact this great herbal doctor via his Email : Priestazibasolutioncenter@gmail.com and WhatsApp Him on +2348100368288

    ReplyDelete
  24. I want to share this wonderful testimony to the Good people all over the world on how I was able to Enlarge my Penis by Dr. Aziba. I was living a shameful life from my young age, just last month as I was browsing on the internet about Penis size and Enlargement Products, I saw a testimony of a Man called Tim James testifying of how he was able to get his penis Enlarged by Dr. Aziba and I decided to also Email Dr. Aziba for my small penis size and he quickly respond to me and gave me the normal instructions which i did and then he shipped the product to me here in the united state which i received in just 3 working days and today i am very happy because i started seeing positive changes in my penis size in just 7 days of use. Dr Aziba herbal product is the best recommended for you and to whom ever suffering from this shame or having any other diseases as well should Contact this great herbal doctor via his Email : Priestazibasolutioncenter@gmail.com and WhatsApp Him on +2348100368288

    ReplyDelete
  25. I am Doctor Paul I got affected with HIV in the process of attending to my HIV patient I tried all I can to get cured but all to no avail, until I saw a post in a health forum about a herbalist man who prepare herbal medication to cure all kind of diseases including HIV virus, at first I doubted if it was real but decided to give it a try, when I contact this herbalist via his email Blessedlovetemple@gmail.com and he prepared a HIV herbal cure and sent it to me via fed-ex delivery company service, when I received this herbal cure, he gave me step by directions on how to apply it, when I applied it as instructed, I was totally cured of this deadly disease within 5 days of usage, I am now free from the deadly disease called HIV, all thanks to Dr Mark. Contact this great herbal spell caster. Kindly contact him. Blessedlovetemple@gmail.com
    He cures all kinds of sickness or diseases such as: 1. HERPES VIRUS 2. LASSA FEVER 3. GONORRHEA 4. HIV/AID 5. EX BACK.
    Thanks Dr Mark for saving my life.

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  26. It's a privilege to share this miraculous testimony to the world. My name is Marianella Cordero, My husband divorced me 8 months back and I have been filled with remorse for I didn't know what to do to amend issues with my husband. I searched for help on the internet on how I could get help in my marriage and I discovered great testifiers about DR JOHN SOCO who has been progressive with his spells. I got in touch with him and behold, DR JOHN SOCO, told me that he will prepare a spell for me that will bring back my husband. I was skeptical but I had no other option but to work with him. 3 days later, my husband called me that he's coming back home and from that day till this moment, we have been living peacefully. He is back now with so much love and caring. today i am glad to let you all know that this spell caster has the power to bring lovers back and the most surprising thing is that our love is very strong now, every day is happiness and joy. and There is nothing like being with the man you love. I will highly recommend DR JOHN SOCO to anyone one out there who needs help whatsoever. If you have any problem contact DR JOHN SOCO, i give you 100% guarantee that he will help you!!. Email: Drjohnsoco@gmail.com or whatsApp number: +1 706 871 4571 ..

    ReplyDelete

  27. I started on COPD Herbal treatment from Ultimate Health Home, the treatment worked incredibly for my lungs condition. I used the herbal treatment for almost 4 months, it reversed my COPD. My severe shortness of breath, dry cough, chest tightness gradually disappeared. Reach Ultimate Health Home via their website www.ultimatelifeclinic.com . I can breath much better and It feels comfortable!

    ReplyDelete
  28. “So I’m someone’s mom!” Welcoming our first child, Cecily , at 12:24 p.m. on Tuesday, July 14. We are absolutely head over heels in love with Cecily, and parenthood is already the most insane and beautiful thing in existence, It's made me excited to have a little spitfire of a daughter of my own. I remembered when i found out i was pregnant 3years ago and was about to walk away from the musical. But at eight weeks, i had a miscarriage. i was so unhappy, until i seek help spiritually from Priest Babaka the herbal practitioner, who helps and guide me to get pregnant again, even at the trying times few weeks in April when i battled symptoms of the coronavirus including "a cough that makes it feel like my head is splitting open from the inside out, but luckily, the baby was okay with the Priest help. i am happy to finally be a mother, couples out there that needs help, trying to conceive a baby, contact Priest Babaka on babaka.wolf@gmail.com or Facebook at priest.babaka , you will definitely have a baby to make you a parent. He handle Pregnancy related cases.

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  29. People actually believe there is no cure for herpes due to what our medical Doctor do always say to us..but now i know that there is a cure for it Through herbal remedy' i and my boyfriend was once haven herpes type 1&2 i contacted Dr Edidia for herbal treatment and he prepared us some herbs which we takes for some couples of days, and we take it as he says before i am to known' i and my boyfriend was perfectly cure for it and never experience the Outbreaks again. please friends if you have herpes virus and you really want to get rid of it permanently, Kindly contact this great Dr Edidia for herbal treatment and you will get rid of it.. truly truly home remedies works perfectly. EMAIL dredidiaherbalhome@gmail.com cell phone contact +2349074505296 his whatsapp contact is +2349074505296.

    ReplyDelete
  30. Hello viewers, I have been a victim of the Herpes virus for 11months. One day i was in a mall when I meant a friend of mine that once knew that i was suffering from same problem with hers and she introduce me to a man called Dr Okorom that have cure her Herpes virus with the help of his traditional medicine I contacted him and he gave me the traditional herbal medicine to drink and after drinking it with his prescription he told me that after 2 weeks I will see the result of his traditional herbal medicine and today I am happy to say that Dr Okorom have used his traditional herbal medicine to cure my Herpes virus, and now I am happy to be alive life again today with my family, I am married with 2 kids, to all that feels my pain and will need to know more about this great traditional doctor here is his contact: dr.okoromspellhome@live.com or WhatsApp him via +33 6 05 81 04 88 i am sure he is the solution you have been waiting for so don't let this opportunity pass you by okay.

    ReplyDelete
  31. I am here to give my testimony
    about Dr babaiba who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by salami, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Dr babaiba cured them. when i contacted him he gave me hope and send a Herbal medicine to me that i took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so happy as i am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr babaiba healing herbal cure of all kinds of sickness you may have like
    #CANCER,
    #DIABETES,
    #HIV
    #AIDS,
    #Herpes
    #Std
    #URINARYTRACT #INFECTION,
    #CANCER,
    #IMPOTENCE,
    #BARENESS
    #INFERTILITY
    #DIARRHEA
    #ASTHMA
    #SIMPLEXHERPES #GENITAL
    #COLDSORE
    . he also cure my friend from cervical #cancer Email: (drbabaibaherbalsolutiontemple@gmail.com) or call or WHATAPP +2348140046509 I am forever greaful ...

    ReplyDelete
  32. I have been with bad mouth Odour and tooth pain for the past 5year..  on till I  saw people testifying about Dr Ishiaku on the great work his has done and I contacted him on WhatsApp and explain to him and he made the herbal medicine for me and I got cured….my mouth doesn’t smell anymore and my tooth are strong &healthy…Big thanks to Dr Ishiaku for his great job. so if you are with any of this diseases

    HIV
    CANCER
    HERPES (HSVHPV ORAL OR GENITAL )
    DIABETICSFYBRIOD

    please contact Dr Ishiaku on WhatsApp +2348180828544

    ReplyDelete

  33. Dear friends online My name is Destiny Hudson And I live in the USA, Dominica I am here today to appreciate the good work of Dr Oyagu, for helping me curing my HSV 1&2 Disease, I contacted the virus from my Partner, i found out he was cheating on me, so i broke up with him, 2 month later i went to test myself in the hospital when i was seeing sore on my mouth and Private areas, i was diagnosed of HSV 1&2. i tried using the acyclovir medicine but it didn't help in curing it, i went on a search and found out Dr Oyagu curing people illness, and so i got his email and i was told how i can get his herbal cure, 3 days later i received his medicine after using it for 2 weeks i was tested again and was confirm negative, today i am totally free. If you are over there looking for solution email: oyaguherbalhome@gmail.com or contact him on whatsapp +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome today and share your own testimony. And He also has herbal cure for the Following DISEASES, eczema, urethra wart, chronic problems. Herpes, Cancer, Als, Hepatitis, Diabetes, HPV ,Infections, ulcer ETC

    ReplyDelete
  34. I want to share to the world how Dr Maggi helped me cure my Herpes with his herbs i never believe it will work because i was introduce to him by my friend who was cure of herpes by Dr Maggi, so i will tell you all who is looking for a cure to their herpes, he took research before he could finally get the solution to it. Thank God today I am a beneficiary to this cure and I went back to the hospital after 14days of taking the herbs and I was tested negative, and he also cures any kind of cancer without any surgery and he heals with natural herbs. please i urge you to contact him now through his email address: drmaggiherbalhome@gmail.com or call/whatsApp him on +19179796387

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  35. Greatest Joy in my life today HIV/AIDS has cure but doctor said HIV Disease has no cure until i met Doctor Kabiki who help me in my life. I was infected with HIV/AIDS DISEASE in 2014, i went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution,so that my body can be okay.until this faithful day as i was browsing the net I saw a testimony on how Doctor Kabiki helped people in curing HIV disease, so i contacted him for solution for my HIV DISEASE,so Doctor Kabiki told me that his going to prepare herbal medicine for my health,then he prepared the medicine and luckily after 3week my HIV we be cured.Doctor Kabiki is well recognize as one of the best herbalist doctor in Africa, you don't have to be sad anymore or share your tears anymore on this disease when the cure have already be found by Doctor Kabiki herbal medicine,he so cure HPV,CANCER,HERPES,ALS,HEPATITIS B, DIABETIC, ASTHMA,contact him via email: drkibakisolutiontemple@gmail.com or whatsapp number : +254778109549.

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  36. I'm recommending Dr Okuofoh to everyone who has the herpes simplex virus to get the cure from him. I was diagnosed with genital herpes in 2020 and I have been searching and asking questions to see if I could get something to cure the disease because I did not believe what the doctors say that no cure is found yet. I came across a comment on Youtube and the person testify how she was cured of herpes after using Dr Okuofoh herbal medicine. I quickly contact Dr Okuofoh and explain my problem to him and he prepares the herbs and send it to me through UPS and gave me instructions on how to use it and tell me to go for a checkup after usage which I did after two weeks of taken the herbal medicine and my result was NEGATIVE. I waited another month and retested the result was still NEGATIVE and my doctor told me that am completely free from herpes. Am so happy and grateful to Dr Okuofoh for what he has done for me and I will continue to share this with people out there to know that there is a herpes cure can contact Dr Okuofoh by email and Whats App to get the cure from him.

    Email: drokuofohherbalhomehealing@gmail.com
    Call or WhatsApp +2349050141449.

    ReplyDelete
  37. Greetings to the general public, I want to tell about how I was cured of
    HIV disease by a Doctor called Dr. voodoo . I was browsing the Internet
    searching for remedy on HIV and I saw a comment of people talking about how
    Doctor voodoo cured them. I was scared because I never believed in the
    Internet but i was convince to give him a try because i having no hope of
    been cured of HIV so I decided to contact him with his email that was
    listed on the comment voodoospelltemple66@gmail.com when I contacted
    him he gave me hope and send a Herbal medicine to me that I took and
    it seriously worked for me, am a free person now without problem,
    my HIV result came out negative. I pray for you Dr. voodoo God will give
    you everlasting life, you shall not die before your time for being sincere
    and great men. Am so happy, you can also contact him if you have any
    problem Email: voodoospelltemple66@gmail.com
    or Add him up on WhatsAp +2348140120719

    ReplyDelete
  38. I was diagnosed of herpes 3 years, and ever since then i have been taking treatment to prevent outbreaks, burning and blisters, but there was no improvement until i came across testimonies of Dr. VOODOO on how he has been curing different people from different diseases all over the world, then i contacted him. After our conversation he sent me the medicine which i took according to his instructions for up to 2 weeks. After completing the medication i went back to my doctor for another test and the virus was all gone and i was completely cured, since then i have not had any signs of outbreak. I'm so filled with joy. With herbal medication Herpes Virus is 100% curable. I refer Dr VOODOO to everyone out there with the virus Email: voodoospelltemple66@gmail.com Add Dr voodoo on whatsApp +2348140120719

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  39. I tested positive for HSV-2 a few months ago. At first, yes, I was devastated thinking I have an incurable STD for life. After doing hours of research I quickly realized genital herpes is a filthy parasite that just likes to hide in your body. Yes, there’s no “medical cure” but that doesn’t mean your body can’t get rid of it. I literally flush it out of my system with the help of Dr. Azu natural herbal medication which I ordered after doing some research. And now I’m HSV-2 Negative, I never believed it until my doctor finally gave me the test results and behold I was HSV free. I’m so glad that I found you Dr. Azu. Thanks so much for your goodness I’m grateful. You can also get your help from him through his Whats App Contact for fast communication +2347087313747 OR  Email:dr.azuherbalhome@gmail.com .

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  40. Join the Illuminati Freemason Brotherhood!!!Email illuminatisecretetemple666@yahoo.com.........

    BENEFITS GIVEN TO NEW MEMBERS WHO JOIN ILLUMINATI.
    1.A Cash reward worth $500,000, 00 after initiation.
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    If you are suffering from Herpes...contact this herbal doctor today on this Email:robinsonbuckler@yahoo.com..

    ReplyDelete
  41. I can’t believe this. A great testimony that i must share to all HERPES patient in the world i never believed that their could be any complete cure for HERPES or any cure for HERPES,i saw people’s testimony on sites of how DR Afrid prepares herbal cure and brought them back to life again. i had to try it too and you can not believe that in just a few weeks i started using it all my pains stopped gradually and i had to leave without the HERPES DRUGS the doctor gave to me. Right now i can tell you that for a few months now i have not had any pain, delay in treatment leads to death. Here is his email:drafridherbalhome@gmail.com or contact on whatsapp at +1(412) 405-1961

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  42. I have suffered from HERPES Simplex Virus and was totally depressed about it until I met Doctor excel the great herbal healer who cured me from the nasty virus by drinking the herbal medicine that he sent me. for more information visit his website to know more about his medication. https://excelherbalcure.com

    ReplyDelete