Please only follow these instructions if:
· The index sequences you have used are visible in the index sequences tab of the submission form
· There are fewer than 384 samples within your pool.If the points above are not true, please see section, unspecified index further on in this blog.
1. Completing the sample/reagent label field
1a. Navigate to the index sequences tab of the sample submission form.
1b. Search for your index sequences
1c. Copy the index name from column C of the index sequences tab, e.g A001-A005 to the column Sample/Reagent Label, of the submission form tab.
Figure 1-index sequences tab of the sample submission form
2. Completing the UDF/Index type field
2a. Select the correct UDF/Index type from the drop down menu on the submission form tab.IMPORTANT –please make sure the Index type field matches column B of the index sequences tab. This ensures that your library goes through our acceptance step. Please see the two following examples.
Example1- I am submitting a Truseq LT library consisting of 5 samples and used indexes A001-A005.The sample/reagent label on the submission form should read A001-A005.
The UDF/index type should read Truseq LT.
Figure 3 - The index type field next to these indexes is Truseq LT so this is what should be entered into the UDF/Index type field.
Figure 4- Submission form
In most cases, the Index type will match to the indexes you have used as expected. However there are now multiple kits available which share the same indexes.
Because of this, there may be some cases where the index sequences you select will have a different Index type to the library you have made. (see example 2 below) This may affect you if you are submitting for Nextera XT or Nextera.
Example 2- I used indexes N701-N501, N702-N501, N703-N501, N704-N501. I prepared the libraries using a Nextera XT library prep kit.
The sample/reagent label on the submission form should read N701-N501, N702-N501, N703-N501, N704-N501. The UDF/index type should read Nextera and not Nextera XT. This is because the UDF/Index type needs to match column B on the index sequences tab.
Figure 5 - The index type field next to these indexes is Nextera so this is what should be entered into the UDF/Index type field
Once you have submitted your libraries, the Genomics Core would like to start working on your sequencing as soon as we can.
If the incorrect index type has been selected, we will need to delete your submission and we would ask you to submit again after making changes to your sample sheet and following the instructions above. Of course whilst this guide should be used to help you, we are always here to discuss this with you in person if you have any questions. Alternatively you can contact us on our helpdesk: firstname.lastname@example.org